Isolation of Extreme Halophiles from Seawater

Isolation of extreme halophiles from seawater.

Extreme halophilic bacteria were isolated from the ocean off the coast of Spain. All were gram-negative cocci. One isolate was compared to Halococcus sp. NCMB 757 and was found to have similar characteristics.

Extreme halophiles synthesize betaine from glycine by methylation.

Glycine betaine is a compatible solute, which is able to restore and maintain osmotic balance of living cells. It is synthesized and accumulated in response to abiotic stress. Betaine acts also as a methyl group donor and has a number of important applications including its use as a feed additive. The known biosynthetic pathways of betaine are universal and very well characterized. A number of ...

Drastic differences in glycosylation of related S-layer glycoproteins from moderate and extreme halophiles.

The outer surface of the moderate halophilic archaebacterium Haloferax volcanii (formerly named Halobacterium volcanii) is covered with a hexagonally packed surface (S) layer glycoprotein. The polypeptide (794 amino acid residues) contains 7 N-glycosylation sites. Four of these sites were isolated as glycopeptides and the structure of one of the corresponding saccharides was determined. Oligosa...

Isolation and biochemical characterization of Halophiles from Sahastradhara region, Dehradun, India

Halophiles are extremophile organisms that thrive in environments with very high concentrations of salt. Halophiles are categorized as slight, moderate or extreme, based on the extent of their halotolerance. Slight halophiles prefer 0.3 to 0.8 M (1.8 to 4.7% seawater is 0.6 M or 3.5%), moderate halophiles 0.8 to 3.4 M (4.7 to 20%), and extreme halophiles 3.4 to 5.1 M (20 to 30%) NaCl. Halophile...

isolation and identification of new polyhydroxyalkanoate (pha) producing bacteria from an extreme environment